PREPARATION OF SPHEROPLASTS FROM VIBRIO COMMA
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چکیده
منابع مشابه
Preparation of Spheroplasts from Vibrio Comma.
Chatterjee, B. R. (Baylor University College of Medicine, Houston, Texas), and Robert P. Williams. Preparation of spheroplasts from Vibrio comma. J. Bacteriol. 85:838-841. 1963.-Spheroplasts were prepared from several strains of Vibrio comma by lysozyme treatment combined with freezing and thawing of the organisms. The optimal concentration of lysozyme was 50 mug/ml, although some spheroplasts ...
متن کاملCulture of serum-induced spheroplasts from Vibrio cholerae.
Spheroplasts of Vibrio cholerae types Inaba, Hikojima, and Ogawa were produced in serum. An excess of lysozyme was added to expedite transformation, and CaCl(2) was used as the stabilizing agent. At the optimal time for each strain, when less than 1% classical rod forms were observed microscopically, samples were plated on both conventional and modified L-form media. No growth occurred on conve...
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Feeley and Pittman (Bull. World Health Organ. 28:347, 1963) speculated that a hemolysin (HL) destructive enzyme produced by El Tor vibrio at a late stage of growth lowers the HL titer. We proved the production of an HL-destructive factor (HDF) by strains of classical cholera and El Tor vibrio and established an in vitro method for quantitative titration of HDF. HL was prepared by growing a hemo...
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(1918) view that they were attached to the cell membrane and third, that of Fuhrmann (1910) who stated that they arose from blepharoplasts. None of these opinions was accompanied by clear cut photographic evidence. The development and use of the electron microscope has made it possible to observe structures which were invisible with the light microscope. Thus Mudd and Anderson (1942) made elect...
متن کاملClassification of Vibrio Cholerae (vibrio Comma), including El Tor Vibrios, by Infrasubspecific Characteristics.
Feeley, John C. (National Institutes of Health, Bethesda, Md.). Classification of Vibrio cholerae (Vibrio comma), including El Tor vibrios, by infrasubspecific characteristics. J. Bacteriol. 89:665-670. 1965.-A study of the properties of 220 serotype O group I vibrios indicated striking similarity in most of their properties. However, by using four tests often applied in the identification of t...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1963
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.85.4.838-841.1963